Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides - Architecture et Fonction des Macromolécules Biologiques Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2022

Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides

Lucelia Cabral
  • Fonction : Auteur
Gabriela Persinoti
  • Fonction : Auteur
Douglas Paixão
  • Fonction : Auteur
Marcele Martins
  • Fonction : Auteur
Mariana Morais
  • Fonction : Auteur
Mariana Chinaglia
  • Fonction : Auteur
Mariane Domingues
  • Fonction : Auteur
Mauricio Sforca
  • Fonction : Auteur
Renan Pirolla
  • Fonction : Auteur
Wesley Generoso
  • Fonction : Auteur
Clelton Santos
  • Fonction : Auteur
Lucas Maciel
  • Fonction : Auteur
Mario Murakami
  • Fonction : Auteur

Résumé

The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the microbial community composition, enzymatic systems and metabolic pathways involved in the conversion of dietary fibers into short-chain fatty acids, a main energy source for the host. In this microbiota, the unconventional enzymatic machinery from Fibrobacteres seems to drive cellulose degradation, whereas a diverse set of carbohydrate-active enzymes from Bacteroidetes, organized in polysaccharide utilization loci, are accounted to tackle complex hemicelluloses typically found in gramineous and aquatic plants. Exploring the genetic potential of this community, we discover a glycoside hydrolase family of β-galactosidases (named as GH173), and a carbohydrate-binding module family (named as CBM89) involved in xylan binding that establishes an unprecedented three-dimensional fold among associated modules to carbohydrate-active enzymes. Together, these results demonstrate how the capybara gut microbiota orchestrates the depolymerization and utilization of plant fibers, representing an untapped reservoir of enzymatic mechanisms to overcome the lignocellulose recalcitrance, a central challenge toward a sustainable and bio-based economy.
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Dates et versions

hal-03589026 , version 1 (06-09-2023)

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Lucelia Cabral, Gabriela Persinoti, Douglas Paixão, Marcele Martins, Mariana Morais, et al.. Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides. Nature Communications, 2022, 13 (1), pp.629. ⟨10.1038/s41467-022-28310-y⟩. ⟨hal-03589026⟩
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