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Article Dans Une Revue AJP - Regulatory, Integrative and Comparative Physiology Année : 2014

Myostatin is a key mediator between energy metabolism and endurance capacity of skeletal muscle

Etienne Mouisel
  • Fonction : Auteur
Karima Relizani
  • Fonction : Auteur
Laurence Mille-Hamard
  • Fonction : Auteur
Raphael Denis
  • Fonction : Auteur
Christophe Hourde
  • Fonction : Auteur
Onnik Agbulut
Ketan Patel
  • Fonction : Auteur
Ludovic Arandel
Susanne Morales-Gonzalez
  • Fonction : Auteur
Alban Vignaud
  • Fonction : Auteur
Luis Garcia
  • Fonction : Auteur
Arnaud Ferry
Serge Luquet
Veronique Billat
  • Fonction : Auteur
  • PersonId : 891825
Renee Ventura-Clapier
  • Fonction : Auteur
Markus Schuelke
Helge Amthor
  • Fonction : Auteur

Résumé

Myostatin (Mstn) participates in the regulation of skeletal muscle size and has emerged as a regulator of muscle metabolism. Here, we hypothesized that lack of myostatin profoundly depresses oxidative phosphorylation-dependent muscle function. Toward this end, we explored Mstn(-/-) mice as a model for the constitutive absence of myostatin and AAV-mediated overexpression of myostatin propeptide as a model of myostatin blockade in adult wild-type mice. We show that muscles from Mstn(-/-) mice, although larger and stronger, fatigue extremely rapidly. Myostatin deficiency shifts muscle from aerobic toward anaerobic energy metabolism, as evidenced by decreased mitochondrial respiration, reduced expression of PPAR transcriptional regulators, increased enolase activity, and exercise-induced lactic acidosis. As a consequence, constitutively reduced myostatin signaling diminishes exercise capacity, while the hypermuscular state of Mstn(-/-) mice increases oxygen consumption and the energy cost of running. We wondered whether these results are the mere consequence of the congenital fiber-type switch toward a glycolytic phenotype of constitutive Mstn (/) mice. Hence, we overexpressed myostatin propeptide in adult mice, which did not affect fiber-type distribution, while nonetheless causing increased muscle fatigability, diminished exercise capacity, and decreased Pparb/d and Pgc1a expression. In conclusion, our results suggest that myostatin endows skeletal muscle with high oxidative capacity and low fatigability, thus regulating the delicate balance between muscle mass, muscle force, energy metabolism, and endurance capacity.

Dates et versions

hal-01545449 , version 1 (22-06-2017)

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Citer

Etienne Mouisel, Karima Relizani, Laurence Mille-Hamard, Raphael Denis, Christophe Hourde, et al.. Myostatin is a key mediator between energy metabolism and endurance capacity of skeletal muscle. AJP - Regulatory, Integrative and Comparative Physiology, 2014, 307 (4), pp.R444-R454. ⟨10.1152/ajpregu.00377.2013⟩. ⟨hal-01545449⟩
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