Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice - Biological Adaptation and Ageing Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2017

Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice

Pascal Challande

Résumé

Intermediate filaments are involved in stress-related cell mechanical properties and in plasticity via the regulation of focal adhesions (FAs) and the actomyosin network. We investigated whether vimentin regulates endothelial cells (ECs) and vascular smooth muscle cells (SMCs) and thereby influences vasomotor tone and arterial stiffness. Vimentin knockout mice (Vim−/−) exhibited increased expression of laminin, fibronectin, perlecan, collagen IV and VE-cadherin as well as von Willebrand factor deposition in the subendothelial basement membrane. Smooth muscle (SM) myosin heavy chain, α-SM actin and smoothelin were decreased in Vim−/− mice. Electron microscopy revealed a denser endothelial basement membrane and increased SM cell-matrix interactions. Integrin αv, talin and vinculin present in FAs were increased in Vim−/− mice. Phosphorylated FA kinase and its targets Src and ERK1/2 were elevated in Vim−/− mice. Knockout of vimentin, but not of synemin, resulted in increased carotid stiffness and contractility and endothelial dysfunction, independently of blood pressure and the collagen/elastin ratio. The increase in arterial stiffness in Vim−/− mice likely involves vasomotor tone and endothelial basement membrane organization changes. At the tissue level, the results show the implication of FAs both in ECs and vascular SMCs in the role of vimentin in arterial stiffening.
Fichier principal
Vignette du fichier
s41598-017-12024-z.pdf (2.24 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01609296 , version 1 (03-10-2017)

Licence

Paternité

Identifiants

Citer

Benoît Langlois, Ekaterina Belozertseva, Ara Parlakian, Mustapha Bourhim, Jacqueline Gao-Li, et al.. Vimentin knockout results in increased expression of sub-endothelial basement membrane components and carotid stiffness in mice. Scientific Reports, 2017, 7 (1), pp.11628. ⟨10.1038/s41598-017-12024-z⟩. ⟨hal-01609296⟩
291 Consultations
260 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More