Rotavirus Infection Induces Cytoskeleton Disorganization in Human Intestinal Epithelial Cells: Implication of an Increase in Intracellular Calcium Concentration - Biological Adaptation and Ageing Accéder directement au contenu
Article Dans Une Revue Journal of Virology Année : 2000

Rotavirus Infection Induces Cytoskeleton Disorganization in Human Intestinal Epithelial Cells: Implication of an Increase in Intracellular Calcium Concentration

Résumé

ABSTRACT Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. In vivo, rotavirus exhibits a marked tropism for the differentiated enterocytes of the intestinal epithelium. In vitro, differentiated and undifferentiated intestinal cells can be infected. We observed that rotavirus infection of the human intestinal epithelial Caco-2 cells induces cytoskeleton alterations as a function of cell differentiation. The vimentin network disorganization detected in undifferentiated Caco-2 cells was not found in fully differentiated cells. In contrast, differentiated Caco-2 cells presented Ca 2+ -dependent microtubule disassembly and Ca 2+ -independent cytokeratin 18 rearrangement, which both require viral replication. We propose that these structural alterations could represent the first manifestations of rotavirus-infected enterocyte injury leading to functional perturbations and then to diarrhea.

Dates et versions

hal-03975591 , version 1 (06-02-2023)

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Jean-Philippe Brunet, Nathalie Jourdan, Jacqueline Cotte-Laffitte, Catherine Linxe, Monique Géniteau-Legendre, et al.. Rotavirus Infection Induces Cytoskeleton Disorganization in Human Intestinal Epithelial Cells: Implication of an Increase in Intracellular Calcium Concentration. Journal of Virology, 2000, 74 (22), pp.10801-10806. ⟨10.1128/jvi.74.22.10801-10806.2000⟩. ⟨hal-03975591⟩
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