Dysregulation of the anti-mullerian hormone system by steroids in women with polycystic ovary syndrome - Unité de Biologie Fonctionnelle et Adaptative
Article Dans Une Revue Journal of Clinical Endocrinology and Metabolism Année : 2017

Dysregulation of the anti-mullerian hormone system by steroids in women with polycystic ovary syndrome

Résumé

Context: Anti-Mullerian hormone (AMH) and AMH type II receptor (AMHR2) are overexpressed in granulosa cells (GCs) from women with polycystic ovary syndrome (PCOS), the most common cause of female infertility. Objective: The aim of the study was to compare the regulation of the AMH/AMHR2 system by 5adihydrotestosterone (5a-DHT) and estradiol (E2) in GCs from control subjects and women with PCOS. Design, Setting, Patients: Experiments were performed on follicular fluids (FF) and GCs from women undergoing in vitro fertilization. Main Outcome Measures: FF steroid levels were measured by mass spectrometry, and messenger RNA (mRNA) accumulation was quantified by reverse transcription real-time polymerase chain reaction. Results: Total testosterone (T), free T, and 5 alpha-DHT FF levels were significantly higher (P < 0.001) in women with PCOS than in controls. However, E2 and sex hormone-binding globulin concentrations were comparable between the two groups. In GCs from control women, the AMH and AMHR2 expression were not affected by 5a-DHT treatment, whereas AMH mRNA levels were upregulated by 5a-DHT in GCs from patients with PCOS (2.3-fold, P < 0.01) overexpressing the androgen receptor (1.4-fold, P < 0.05). E2 downregulated the AMH and AMHR2 expression in GCs from control women (1.4-fold, P < 0.001 and 1.8-fold, P < 0.01, respectively) but had no effect on these genes in GCs from women with PCOS. This differential effect of E2 was associated with a higher estrogen receptor 1 expression in GCs from women with PCOS (1.9-fold, P < 0.05). Conclusions: In GCs from women with PCOS, the regulation of AMH and AMHR2 expression is altered in a way that promotes the overexpression of the AMH/AMHR2 system, and could contribute to the follicular arrest observed in these patients.

Dates et versions

hal-02628227 , version 1 (26-05-2020)

Identifiants

Citer

Alice Pierre, Joëlle Taieb, Frank Giton, Michael Grynberg, Salma Touleimat, et al.. Dysregulation of the anti-mullerian hormone system by steroids in women with polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 2017, 102 (11), pp.3970-3978. ⟨10.1210/jc.2017-00308⟩. ⟨hal-02628227⟩
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