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Journal Articles Frontiers in Oncology Year : 2023

Defining biomarkers in oral cancer according to smoking and drinking status

Ioannis Karagiannidis
  • Function : Author
Claude Baillou
  • Function : Author
Lisa Belin
  • Function : Author
Maude Guillot-Delost
  • Function : Author
Rodney Macedo
  • Function : Author
Aline Le Moignic
  • Function : Author
Véronique Mateo
  • Function : Author
Patrick Soussan
Isabelle Brocheriou
  • Function : Author
Jean-Luc Teillaud
  • Function : Author
Marie-Caroline Dieu-Nosjean
  • Function : Author
Chloé Bertolus
  • Function : Author
Francois Michel Lemoine
  • Function : Author
Géraldine Lescaille
  • Function : Author

Abstract

Introduction Oral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventually associated to alcohol (Smoker/Drinker patients: SD), but 25-30% of the patients have no identified risk factors (Non-Smoker/Non-Drinker patients: NSND). We hypothesized that these patients have distinguishable immune profiles that could be useful for prognosis. Materials and Methods Cells present in immune tumor microenvironment (TME) and blood from 87 OSCC HPV-negative patients were analyzed using a multiparameter flow cytometry assay, in a prospective case-control study. Cytokine levels in tumor supernatants and blood were determined by a cytometric bead array (CBA) assay. Results Normal gingiva and blood from healthy donors (HD) were used as controls. A significant increase of granulocytes (p<0.05 for blood), of monocytes-macrophages (p<0.01 for blood) and of CD4 + T cells expressing CD45RO and CCR6 (p<0.001 for blood; p<0.0001 for TME) as well as higher levels of IL-6 (p<0.01 for sera, p<0.05 for tumor supernatant) were observed in SD patients as compared to NSND OSCC patients and HD. High percentages of CD4 + T cells expressing CD45RO and CCR6 cells in tumor tissue (p=0.05) and blood (p=0.05) of SD OSCC patients were also associated with a poorer prognosis while a high percentage of regulatory T cells (Treg) in tumor tissue was associated with a more favorable prognostic factor (p=0.05). Also, a higher percentage of blood CD8 + T lymphocytes among CD45 + cells in NSND patients was associated with a better disease-free survival (p=0.004). Conclusion Granulocytes, monocytes-macrophages, and CD4 + T cells expressing CD45RO and CCR6 in blood and TME as well as serum IL-6 can therefore distinguish OSCC SD and NSND patients. Quantifying the proportion of CD4 + T cells expressing CD45RO and CCR6 and of Treg in SD patients and CD8 + T cells in NSND patients could help defining the prognostic of OSCC patients.
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Dates and versions

inserm-03969838 , version 1 (02-02-2023)

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Juliette Rochefort, Ioannis Karagiannidis, Claude Baillou, Lisa Belin, Maude Guillot-Delost, et al.. Defining biomarkers in oral cancer according to smoking and drinking status. Frontiers in Oncology, 2023, 12, ⟨10.3389/fonc.2022.1068979⟩. ⟨inserm-03969838⟩
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