Marseillevirus-like virus recovered from blood donated by asymptomatic humans. - Centre de recherche en cancérologie de Lyon (UMR INSERM 1052 ; CNRS 5286 ; Centre Léon Bérard) Accéder directement au contenu
Article Dans Une Revue Journal of Infectious Diseases Année : 2013

Marseillevirus-like virus recovered from blood donated by asymptomatic humans.

Résumé

The study of the human virome is still in its infancy, especially with regard to the viral content of the blood of people who are apparently disease free. In this study, the genome of a new giant virus that is related to the amoeba-infecting pathogen Marseillevirus was recovered from donated blood, using high-throughput sequencing. Viral antigens were identified by an immunoconversion assay. The virus was visualized with transmission electron microscopy and fluorescence in situ hybridization and was grown in human T lymphocytes. Specific antibody reactions were used to identify viral proteins in blood specimens from polymerase chain reactive-positive donors. Finally, we tested 20 blood specimens from additional donors. Three had antibodies directed against this virus, and 2 had circulating viral DNA. This study shows that giant viruses, which are missed by the use of ultrafilters, are part of the human blood virome. The putative pathogenic role of giant viruses in humans remains undefined.

Domaines

Hématologie

Dates et versions

hal-00870828 , version 1 (08-10-2013)

Identifiants

Citer

Nikolay Popgeorgiev, Mickaël Boyer, Laura Fancello, Sonia Monteil, Catherine Robert, et al.. Marseillevirus-like virus recovered from blood donated by asymptomatic humans.. Journal of Infectious Diseases, 2013, 208 (7), pp.1042-50. ⟨10.1093/infdis/jit292⟩. ⟨hal-00870828⟩
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