The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis. - Centre de recherche en cancérologie de Lyon (UMR INSERM 1052 ; CNRS 5286 ; Centre Léon Bérard) Accéder directement au contenu
Article Dans Une Revue Biomaterials Année : 2014

The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis.

Résumé

Several chemokines are important in muscle myogenesis and in the recruitment of muscle precursors during muscle regeneration. Among these, the SDF-1α chemokine (CXCL12) is a potent chemoattractant known to be involved in muscle repair. SDF-1α was loaded in polyelectrolyte multilayer films made of poly(L-lysine) and hyaluronan to be delivered locally to myoblast cells in a matrix-bound manner. The adsorbed amounts of SDF-1α were tuned over a large range from 100 ng/cm(2) to 5 μg/cm(2), depending on the initial concentration of SDF-1α in solution, its pH, and on the film crosslinking extent. Matrix-bound SDF-1α induced a striking increase in myoblast spreading, which was revealed when it was delivered from weakly crosslinked films. It also significantly enhanced cell migration in a dose-dependent manner, which again depended on its presentation by the biopolymeric film. The low-crosslinked film was the most efficient in boosting cell migration. Furthermore, matrix-bound SDF-1α also increased the expression of myogenic markers but the fusion index decreased in a dose-dependent manner with the adsorbed amount of SDF-1α. At high adsorbed amounts of SDF-1α, a large number of Troponin T-positive cells had only one nucleus. Overall, this work reveals the importance of the presentation mode of SDF-1α to emphasize its effect on myogenic processes. These films may be further used to provide insight into the role of SDF-1α presented by a biomaterial in physiological or pathological processes.

Dates et versions

hal-01053349 , version 1 (30-07-2014)

Identifiants

Citer

Fabien Dalonneau, Xi Qiu Liu, Rabia Sadir, Jorge Almodovar, Hichem Mertani, et al.. The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis.. Biomaterials, 2014, 35 (15), pp.4525-35. ⟨10.1016/j.biomaterials.2014.02.008⟩. ⟨hal-01053349⟩
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