Biophysical Dissection of Isolated GPCRs: The Adenosine A2A Receptor under the Bistouries - Centre de recherche en cancérologie de Lyon (UMR INSERM 1052 ; CNRS 5286 ; Centre Léon Bérard) Accéder directement au contenu
Article Dans Une Revue Receptors and Channels Année : 2023

Biophysical Dissection of Isolated GPCRs: The Adenosine A2A Receptor under the Bistouries

Barbara Calamini
Gavin Ferguson
Thierry Langer
Miklos Nyerges
Magali Schwob
  • Fonction : Auteur
Claudia Zilian-Stohrer
  • Fonction : Auteur

Résumé

In an effort to provide an overview of the biophysical approaches used to study G-protein-coupled receptors, we chose to consider the adenosine A2A receptor as a model, as it is widely reported in the literature to explore the way GPCRs are studied nowadays. After a brief introduction of the receptor, we gathered descriptions of the various tools used to investigate the pharmacology and structure of the A2A receptor. We began by describing the key developments which have led to successful studies of GPCRs including the cloning, expression and purification of A2A, and the subsequent characterizations including quality control, binding and functional studies that have been necessary for the further understanding of the receptor. Then, we reviewed the reconstitution of A2A into nanodiscs as well as the use of this biological material in structural mass spectrometry, NMR, calorimetry and various other approaches to gain not only information about the structure and function of A2A, but also the dynamics of the receptor and the tools necessary to pursue such investigations. The body of techniques presented herein are applicable to all GPCRs amenable to purification.
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Dates et versions

hal-04003751 , version 1 (24-02-2023)

Identifiants

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Jean-Louis Banères, Thomas Botzanowski, Jean A Boutin, Barbara Calamini, Jérôme Castel, et al.. Biophysical Dissection of Isolated GPCRs: The Adenosine A2A Receptor under the Bistouries. Receptors and Channels, 2023, 2 (1), pp.47-92. ⟨10.3390/receptors2010004⟩. ⟨hal-04003751⟩
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