Spectral Induced Polarization Response of Bacteria Growth and Decay in Soil Column Experiments - Environnement, Dynamiques et Territoires de la Montagne
Article Dans Une Revue Journal of Geophysical Research: Biogeosciences Année : 2024

Spectral Induced Polarization Response of Bacteria Growth and Decay in Soil Column Experiments

Yalin Song
Xiaoqing Shi
Ahmad Ghorbani
  • Fonction : Auteur
Siyuan Qiang
Kun Xing
  • Fonction : Auteur
Xueyuan Kang
  • Fonction : Auteur
Qilin Wang
Jichun Wu

Résumé

Spectral induced polarization (SIP) exhibits potential to be a nonintrusive approach to monitor bacterial activity in biological hotspots associated with the critical zone of the earth. The polarization of bacteria in a low-frequency electrical field is related to the polarization of their electrical double layer coating their surface. However, few studies have quantified the induced polarization responses on both gram-negative (GN) and gram-positive (GP) bacteria in soil column experiments. To address this gap, 17 experiments using two strains, Pseudomonas aeruginosa O1 (PAO1, GN) and Brevibacillus centrosporus (L3, GP) are conducted. Complex conductivity spectra are collected in the frequency range 10 mHz-10 kHz during bacterial growth and decay phases in soils. The complex conductivity spectra are fitted using a double Cole-Cole model to remove the effect of Maxwell-Wagner polarization. The change in the magnitude of the polarization (quadrature conductivity or normalized chargeability of the low-frequency contribution) is linearly related to the bacterial density, regardless of the type of bacteria. The changes in the normalized chargeability and Cole-Cole relaxation time are directly proportional to the density of bacteria. Furthermore, it is inferred that the thickness of microcolonies plays a critical role in the relaxation time rather than the diameter of individual bacteria. This study expands the potential of SIP for in situ monitoring of microbial activity in soils.

Plain Language Summary Microorganisms are ubiquitous in the environment. Understanding their impact on the critical zone is crucial in the context of climate change. Spectral induced polarization is a geophysical method that has been proven to be sensitive to the presence of bacteria in porous media, but no specific works have been done for both gram-negative and gram-positive bacteria in soils. Column reactor experiments are conducted using the same soil, inoculated with either gram-negative or gram-positive strains. Experimental results indicate a direct proportionality between the magnitude of the low-frequency electrical polarization of the soil and the density of bacteria. It is inferred that the size of microcolonies plays a critical role in the value of the relaxation time. This study provides new quantitative insights into the relationship between the electrical properties and bacterial activity in soils with applications to the nonintrusive monitoring of biological hotspots in the critical zone of the earth.

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hal-04860139 , version 1 (08-01-2025)

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Yalin Song, Xiaoqing Shi, André Revil, Ahmad Ghorbani, Siyuan Qiang, et al.. Spectral Induced Polarization Response of Bacteria Growth and Decay in Soil Column Experiments. Journal of Geophysical Research: Biogeosciences, 2024, 129 (12), pp.e2024JG008050. ⟨10.1029/2024jg008050⟩. ⟨hal-04860139⟩
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