ISDra2 transposition in Deinococcus radiodurans is downregulated by TnpB. - I2BC Accéder directement au contenu
Article Dans Une Revue Molecular Microbiology Année : 2013

ISDra2 transposition in Deinococcus radiodurans is downregulated by TnpB.

Résumé

Transposable elements belonging to the recently identified IS200/IS605 family radically differ from classical insertion sequences in their transposition mechanism by strictly requiring single-stranded DNA substrates. This IS family includes elements encoding only the transposase (TnpA), and others, like ISDra2 from Deinococcus radiodurans, which contain a second gene, tnpB, dispensable for transposition and of unknown function to date. Here, we show that TnpB has an inhibitory effect on the excision and insertion steps of ISDra2 transposition. This inhibitory action of TnpB was maintained when ISDra2 transposition was induced by γ-irradiation of the host cells and required the integrity of its putative zinc finger motif. We also demonstrate the negative role of TnpB when ISDra2 transposition was monitored in a heterologous Escherichia coli host, indicating that TnpB-mediated inhibition does not involve Deinococcus-specific factors. TnpB therefore appears to play a regulatory role in ISDra2 transposition.

Dates et versions

hal-00822757 , version 1 (15-05-2013)

Identifiants

Citer

Cécile Pasternak, Rémi Dulermo, Bao Ton-Hoang, Robert Debuchy, Patricia Siguier, et al.. ISDra2 transposition in Deinococcus radiodurans is downregulated by TnpB.. Molecular Microbiology, 2013, 88 (2), pp.443-55. ⟨10.1111/mmi.12194⟩. ⟨hal-00822757⟩
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