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Article Dans Une Revue Nature Structural and Molecular Biology Année : 2019

Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1

Résumé

Cytochrome c oxidase (complex IV, CIV) is known in mammals to exist independently or in association with other respiratory proteins to form supercomplexes (SCs). In Saccharomyces cerevisiae, CIV is found solely in an SC with cytochrome bc1 (complex III, CIII). Here, we present the cryogenic electron microscopy (cryo-EM) structure of S. cerevisiae CIV in a III2IV2 SC at 3.3 Å resolution. While overall similarity to mammalian homologs is high, we found notable differences in the supernumerary subunits Cox26 and Cox13; the latter exhibits a unique arrangement that precludes CIV dimerization as seen in bovine. A conformational shift in the matrix domain of Cox5A-involved in allosteric inhibition by ATP-may arise from its association with CIII. The CIII-CIV arrangement highlights a conserved interaction interface of CIII, albeit one occupied by complex I in mammalian respirasomes. We discuss our findings in the context of the potential impact of SC formation on CIV regulation.

Dates et versions

hal-02171150 , version 1 (02-07-2019)

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Citer

Andrew M. Hartley, Natalya Lukoyanova, Yunyi Zhang, Alfredo Cabrera-Orefice, Susanne Arnold, et al.. Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome bc1. Nature Structural and Molecular Biology, 2019, 26 (1), pp.78--83. ⟨10.1038/s41594-018-0172-z⟩. ⟨hal-02171150⟩
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