High-resolution transcription maps reveal the widespread impact of roadblock termination in yeast - I2BC Accéder directement au contenu
Article Dans Une Revue EMBO Journal Année : 2018

High-resolution transcription maps reveal the widespread impact of roadblock termination in yeast

Résumé

Transcription termination delimits transcription units but also plays important roles in limiting pervasive transcription. We have previously shown that transcription termination occurs when elongating RNA polymerase II (RNAPII) collides with the DNA-bound general transcription factor Reb1. We demonstrate here that many different DNA-binding proteins can induce termination by a similar roadblock (RB) mechanism. We generated high-resolution transcription maps by the direct detection of RNAPII upon nuclear depletion of two essential RB factors or when the canonical termination pathways for coding and non-coding RNAs are defective. We show that RB termination occurs genomewide and functions independently of (and redundantly with) the main transcription termination pathways. We provide evidence that transcriptional readthrough at canonical terminators is a significant source of pervasive transcription, which is controlled to a large extent by RB termination. Finally, we demonstrate the occurrence of RB termination around centromeres and tRNA genes, which we suggest shields these regions from RNAPII to preserve their functional integrity.

Dates et versions

hal-02176991 , version 1 (08-07-2019)

Identifiants

Citer

Tito Candelli, Drice Challal, Jean-Baptiste Briand, Jocelyne Boulay, Odil Porrua, et al.. High-resolution transcription maps reveal the widespread impact of roadblock termination in yeast. EMBO Journal, 2018, ⟨10.15252/embj.201797490⟩. ⟨hal-02176991⟩
59 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More