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Article Dans Une Revue Nucleic Acids Research Année : 2013

Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo

Thierry Lacombe
  • Fonction : Auteur
Siew Lay Poh
  • Fonction : Auteur
Régine Barbey
  • Fonction : Auteur
Laurent Kuras
  • Fonction : Auteur
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Résumé

Mediator is a prominent multisubunit coactivator that functions as a bridge between gene-specific activators and the basal RNA polymerase (Pol) II initiation machinery. Here, we study the poorly documented role of Mediator in basal, or activatorindependent, transcription in vivo. We show that Mediator is still present at the promoter when the Pol II machinery is recruited in the absence of an activator, in this case through a direct fusion between a basal transcription factor and a heterologous DNA binding protein bound to the promoter. Moreover, transcription resulting from activatorindependent recruitment of the Pol II machinery is impaired by inactivation of the essential Mediator subunit Med17 due to the loss of Pol II from the promoter. Our results strongly support that Mediator is an integral component of the minimal machinery essential in vivo for stable Pol II association with the promoter.
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Dates et versions

hal-03448814 , version 1 (25-11-2021)

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Thierry Lacombe, Siew Lay Poh, Régine Barbey, Laurent Kuras. Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo. Nucleic Acids Research, 2013, 41 (21), pp.9651 - 9662. ⟨10.1093/nar/gkt701⟩. ⟨hal-03448814⟩
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