A three-dimensional perspective on exon binding by a group II self-splicing intron - Archive ouverte HAL Access content directly
Journal Articles EMBO Journal Year : 2000

A three-dimensional perspective on exon binding by a group II self-splicing intron

Abstract

We have used chemical footprinting, kinetic dissection of reactions and comparative sequence analysis to show that in self-splicing introns belonging to subgroup IIB, the sites that bind the 5' and 3' exons are connected to one another by tertiary interactions. This unanticipated arrangement, which contrasts with the direct covalent linkage that prevails in the other major subdivision of group II (subgroup IIA), results in a unique three-dimensional architecture for the complex between the exons, their binding sites and intron domain V. A key feature of the modeled complex is the presence of several close contacts between domain V and one of the intron-exon pairings. These contacts, whose existence is supported by hydroxyl radical footprinting, provide a structural framework for the known role of domain V in catalysis and its recently demonstrated involvement in binding of the 5' exon.
Fichier principal
Vignette du fichier
Costa et al, EMBO J, 2000.pdf (3.2 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03914680 , version 1 (27-01-2023)

Identifiers

Cite

Maria Costa, François Michel, Eric Westhof. A three-dimensional perspective on exon binding by a group II self-splicing intron. EMBO Journal, 2000, 19, pp.5007 - 5018. ⟨10.1093/emboj/19.18.5007⟩. ⟨hal-03914680⟩
1 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More