Article Dans Une Revue Development (Cambridge, England) Année : 2024

Early autonomous patterning of the anteroposterior axis in gastruloids

Kerim Anlaş
Nicola Gritti
Fumio Nakaki
Laura Salamó Palau
David Oriola
Krisztina Arató
Jia Le Lim
Vikas Trivedi

Résumé

Minimal in vitro systems composed of embryonic stem cells (ESCs) have been shown to recapitulate the establishment of the anteroposterior (AP) axis. In contrast to the native embryo, ESC aggregatessuch as gastruloidscan break symmetry, which is demarcated by polarization of the mesodermal marker T, autonomously without any localized external cues. However, associated earliest patterning events, such as the spatial restriction of cell fates and concomitant transcriptional changes, remain poorly understood. Here, we dissect the dynamics of AP axis establishment in mouse gastruloids, particularly before external Wnt stimulation. Through single-cell RNA sequencing, we identify key cell state transitions and the molecular signatures of T + and T -populations underpinning AP polarization. We also show that this process is robust to modifications of aggregate size. Finally, transcriptomic comparison with the mouse embryo indicates that gastruloids develop similar mesendodermal cell types, despite initial differences in their primed pluripotent populations, which adopt a more mesenchymal state in lieu of an epiblast-like transcriptome. Hence, our findings suggest the possibility of alternate ESC states in vivo and in vitro that can converge onto similar cell fates.
Fichier principal
Vignette du fichier
Early autonomous patterning of the anteroposterior axis in gastruloids.pdf (11.4 Mo) Télécharger le fichier
Origine Publication financée par une institution
licence

Dates et versions

hal-04885885 , version 1 (14-01-2025)

Licence

Identifiants

Citer

Kerim Anlaş, Nicola Gritti, Fumio Nakaki, Laura Salamó Palau, Sham Leilah Tlili, et al.. Early autonomous patterning of the anteroposterior axis in gastruloids. Development (Cambridge, England), 2024, 151, ⟨10.1242/dev.202171⟩. ⟨hal-04885885⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More