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Article Dans Une Revue Biodegradation Année : 2004

Evidence of metyrapone reduction by two Mycobacterium strains shown by 1H NMR

Résumé

In situ 1H NMR monitoring ofmetyrapone incubations with resting-cellsof two strains of Mycobacterium,Mycobacterium aurum MO1 andMycobacterium sp. RP1, showed thebiotransformation of this compound,and more precisely the carbonyl-reductionof metyrapone into the correspondingalcohol, metyrapol. This reduction produced bothenantiomers. The use of inhibitorsallowed us to show the multiple enzymaticactivities involved in this biotransformationincluding carbonyl reductase (EC 1.1.1.184)from the short-chain dehydrogenasesuperfamily and aldehyde reductase(EC 1.1.1.2) from the aldo-keto reductasesuperfamily
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Dates et versions

hal-00136049 , version 1 (12-03-2007)

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Bruno Combourieu, Pascale Pascale Besse, P. Besse-Hoggan Besse-Hoggan, Martine Sancelme, Edmund Maser, A.M. Delort. Evidence of metyrapone reduction by two Mycobacterium strains shown by 1H NMR. Biodegradation, 2004, 15, pp.125-132. ⟨10.1023/B:BIOD.0000015625.94368.a5⟩. ⟨hal-00136049⟩
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