In vivo characterization of dynein-driven nanovectors using Drosophila oocytes. - Institut de Chimie de Nice (ICN) - Université Côte d'Azur Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2013

In vivo characterization of dynein-driven nanovectors using Drosophila oocytes.

Résumé

Molecular motors transport various cargoes including vesicles, proteins and mRNAs, to distinct intracellular compartments. A significant challenge in the field of nanotechnology is to improve drug nuclear delivery by engineering nanocarriers transported by cytoskeletal motors. However, suitable in vivo models to assay transport and delivery efficiency remain very limited. Here, we develop a fast and genetically tractable assay to test the efficiency and dynamics of fluospheres (FS) using microinjection into Drosophila oocytes coupled with time-lapse microscopy. We designed dynein motor driven FS using a collection of dynein light chain 8 (LC8) peptide binding motifs as molecular linkers and characterized in real time the efficiency of the FS movement according to its linker's sequence. Results show that the conserved LC8 binding motif allows fast perinuclear nanoparticle's accumulation in a microtubule and dynein dependent mechanism. These data reveal the Drosophila oocyte as a new valuable tool for the design of motor driven nanovectors.

Dates et versions

hal-02545531 , version 1 (17-04-2020)

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Citer

Nadège Parassol, Céline Bienvenu, Cécile Boglio, Sébastien Fiorucci, Delphine Cerezo, et al.. In vivo characterization of dynein-driven nanovectors using Drosophila oocytes.. PLoS ONE, 2013, 8 (12), pp.e82908. ⟨10.1371/journal.pone.0082908⟩. ⟨hal-02545531⟩
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