Localization and characterization of interleukin-1 receptors in the islets of Langerhans from control and nonobese diabetic mice. - BIG - Chimie et Biologie des Métaux (CBM) Accéder directement au contenu
Article Dans Une Revue Endocrinology Année : 1995

Localization and characterization of interleukin-1 receptors in the islets of Langerhans from control and nonobese diabetic mice.

Résumé

Numerous in vivo and in vitro studies have shown the effects of interleukin-1 (IL-1) on insulin and glucagon secretion. To understand the mechanism of these effects, we performed localization and characterization of IL-1 receptors (IL-1R) in pancreas using a quantitative autoradiography method and recombinant human (rh) [125I]IL-1 alpha as a ligand. Frozen sections of pancreas were studied in control (C3H/He) and nonobese diabetic (NOD) mice (a model of autoimmune type I diabetes). Compared to splenic IL-1R, a very high density of specific IL-1R (> 4-fold that in spleen) was found on the islets of Langerhans in both strains. In C3H/He mice, competition experiments demonstrated the presence of one high affinity binding site (Ki = 3.4 and 3.2 x 10(-10) M; binding capacity, 137 and 122 fmol/mg protein for rhIL-1 alpha and rhIL-1 beta, respectively), comparable to type I IL-1R described on T-lymphocytes. In prediabetic NOD mice, these IL-1R were expressed with the same density, affinity, and specificity as in the control strain. Before the onset of diabetes, the expression of IL-1R protein on the islet cells appears to be entirely normal. In contrast, in diabetic NOD mice, IL-1R are sharply decreased, correlating with the intensity of islet destruction. In conclusion, the localization and high density of IL-1R on the mouse islets of Langerhans complement previous studies showing the presence of messenger RNA for type I IL-1R on the islets of Langerhans. These results support a direct physiological effect of IL-1 on pancreatic hormones, such as insulin and glucagon, and a potential role of IL-1R in the pathogenesis of type I diabetes.

Domaines

Neurobiologie
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Dates et versions

hal-00405263 , version 1 (20-07-2009)

Identifiants

  • HAL Id : hal-00405263 , version 1
  • PUBMED : 7835294

Citer

M. Jafarian-Tehrani, A. Amrani, F. Homo-Delarche, C. Marquette, M. Dardenne, et al.. Localization and characterization of interleukin-1 receptors in the islets of Langerhans from control and nonobese diabetic mice.. Endocrinology, 1995, 136 (2), pp.609-13. ⟨hal-00405263⟩
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