Autoradiographic study of neurosteroid binding sites labelled with [35S]-TBPS in brain of different species. - BIG - Chimie et Biologie des Métaux (CBM) Accéder directement au contenu
Article Dans Une Revue Brain Research Bulletin Année : 1993

Autoradiographic study of neurosteroid binding sites labelled with [35S]-TBPS in brain of different species.

Résumé

Distribution of [35S]-TBPS binding sites was studied in various structures of brain in mouse and guinea pig and in cortex of monkey and in hippocampus of postmortem human brain. As it is observed for rat brain, high densities of [35S]-TBPS binding sites were found in layer IV of cortex in the four species, and in thalamus of mouse and guinea pig. Intermediate densities of binding sites were observed in superficial and deep layers of cortex in those four species and in hippocampus of mouse, guinea pig, and human. In all brain structures studied, 5 alpha 3 alpha P and picrotoxin produced a dose-dependent inhibition of [35S]-TBPS binding. No significant interregion or interspecies differences could not be detected for IC50 values of 5 alpha 3 alpha P or picrotoxin to inhibit [35S]-TBPS from its binding sites. In all regions studied, IC50 values were close to 1.5 x 10(-6) M for 5 alpha 3 alpha P and 2.3 x 10(-7) M for picrotoxin.

Domaines

Neurobiologie

Dates et versions

hal-00405275 , version 1 (20-07-2009)

Identifiants

Citer

Monique Vincens, Evelyne Dartois, Marc Popelier, C. M. Marquette, Emmanuel Moyse, et al.. Autoradiographic study of neurosteroid binding sites labelled with [35S]-TBPS in brain of different species.. Brain Research Bulletin, 1993, 32 (3), pp.251-6. ⟨10.1016/0361-9230(93)90184-D⟩. ⟨hal-00405275⟩
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