Soybean lipoxygenases-1, -2a, -2b and -2c do not contain PQQ. - BIG - Chimie et Biologie des Métaux (CBM) Accéder directement au contenu
Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 1990

Soybean lipoxygenases-1, -2a, -2b and -2c do not contain PQQ.

Régis Daniel
C. Chopard
  • Fonction : Auteur
D. Mansuy
  • Fonction : Auteur
C. Cucurou
  • Fonction : Auteur
V. Ullrich
  • Fonction : Auteur
J. C. Chottard
  • Fonction : Auteur

Résumé

Soybean isoenzymes lipoxygenases-1, -2a, -2b and -2c were examined spectroscopically for the presence of covalently bound pyrrolo quinoline quinone (PQQ) after derivatization by phenylhydrazine (PH), 2,4-dinitrophenylhydrazine (DNPH) and 3-methyl-2-benzothiazolinone hydrazone (MBTH). DNPH derivatization of PQQ after a pronase digestion step of lipoxygenase-1 in the presence of an anion exchange gel fixing the cofactor was also investigated. None of these experiments provided evidence for the presence of PQQ contrary to previous report by Van der Meer et al (1). We have checked, by EPR spectroscopy, that the three reactants used were able to reduce the active site ferric iron. Our results were confirmed by the absence of enzyme inhibition by cis- and trans-1,2-diaminocyclohexane or benzylamine in the presence of NaBH3CN which have been reported to react with PQQ and to inactivate quinoproteins (2,3).

Dates et versions

hal-01063007 , version 1 (11-09-2014)

Identifiants

Citer

I. Michaud-Soret, Régis Daniel, C. Chopard, D. Mansuy, C. Cucurou, et al.. Soybean lipoxygenases-1, -2a, -2b and -2c do not contain PQQ.. Biochemical and Biophysical Research Communications, 1990, 172 (3), pp.1122-8. ⟨10.1016/0006-291X(90)91563-8⟩. ⟨hal-01063007⟩
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