Identification of iron(III) peroxo species in the active site of the superoxide reductase SOR from Desulfoarculus baarsii. - BIG - Chimie et Biologie des Métaux (CBM) Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2002

Identification of iron(III) peroxo species in the active site of the superoxide reductase SOR from Desulfoarculus baarsii.

Résumé

The active site of superoxide reductase SOR consists of an Fe2+ center in an unusual [His4 Cys1] square-pyramidal geometry. It specifically reduces superoxide to produce H2O2. Here, we have reacted the SOR from Desulfoarculus baarsii directly with H2O2. We have found that its active site can transiently stabilize an Fe3+-peroxo species that we have spectroscopically characterized by resonance Raman. The mutation of the strictly conserved Glu47 into alanine results in a stabilization of this Fe3+-peroxo species, when compared to the wild-type form. These data support the hypothesis that the reaction of SOR proceeds through such a Fe3+-peroxo intermediate. This also suggests that Glu47 might serve to help H2O2 release during the reaction with superoxide.
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Dates et versions

hal-01075797 , version 1 (20-10-2014)

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Christelle Mathé, Tony A Mattioli, Olivier Horner, Murielle Lombard, Jean-Marc Latour, et al.. Identification of iron(III) peroxo species in the active site of the superoxide reductase SOR from Desulfoarculus baarsii.. Journal of the American Chemical Society, 2002, pp.4966-7. ⟨hal-01075797⟩
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