Small angle neutron scattering contrast variation reveals heterogeneities of interactions in protein gels - Laboratoire Charles Coulomb (L2C) Accéder directement au contenu
Article Dans Une Revue Soft Matter Année : 2016

Small angle neutron scattering contrast variation reveals heterogeneities of interactions in protein gels

Résumé

The structure of model gluten protein gels prepared in ethanol/water is investigated by small angle X-ray (SAXS) and neutrons (SANS) scattering. We show that gluten gels display radically different SAXS and SANS profiles when the solvent is (at least partially) deuterated. The detailed analysis of the SANS signal as a function of the solvent deuteration demonstrates heterogeneities of sample deuteration at different length scales. The progressive exchange between the protons (H) of the proteins and the deuteriums (D) of the solvent is inhomogeneous and 60 nm large zones that are enriched in H are evidenced. In addition, at low protein concentration, in the sol state, solvent deuteration induces a liquid/liquid phase separation. Complementary biochemical and structure analyses show that the denser protein phase is more protonated and specifically enriched in glutenin, the polymeric fraction of gluten proteins. These findings suggest that the presence of H-rich zones in gluten gels would arise from the preferential interaction of glutenin polymers through a tight network of non-exchangeable intermolecular hydrogen bonds.
Fichier principal
Vignette du fichier
Banc-2016-Soft Matter.pdf (1.8 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01317647 , version 1 (18-05-2016)

Identifiants

Citer

Amélie Banc, Céline Charbonneau, Mohsen Dahesh, Marie-Sousai Appavou, Zhendong Fu, et al.. Small angle neutron scattering contrast variation reveals heterogeneities of interactions in protein gels. Soft Matter, 2016, 12 (24), pp.5340-5352. ⟨10.1039/c6sm00710d⟩. ⟨hal-01317647⟩
232 Consultations
166 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More