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Communication Dans Un Congrès Année : 2014

Architecture of a bacterial DNA segregation apparatus: active caging of ParB by stochastic self-assembly nucleated from the centromere

Jean-Charles Walter

Résumé

Many canonical processes in molecular biology rely on the dynamic assembly of higher-order nucleoprotein complexes. In bacteria, the assembly mechanism of ParABS, the nucleoprotein super-complex that actively segregates the bacterial chromosome and many plasmids, remains elusive. We combined super-resolution microscopy, quantitative genome-wide surveys, biochemistry, and mathematical modeling to investigate the assembly of ParB at the centromere-like sequences parS. We found that nearly all ParB molecules are actively confined around parS by a network of synergistic protein-protein and protein-DNA interactions. Interrogation of the empirically determined, high-resolution ParB genomic distribution with modeling suggests that instead of binding only to specific sequences and subsequently spreading, ParB binds stochastically around parS over long distances. We propose a new model for the formation of the ParABS partition complex based on nucleation and caging: ParB forms a dynamic lattice with the DNA around parS. This assembly model and approach to characterizing large-scale, dynamic interactions between macromolecules may be generalizable to many unrelated machineries that self-assemble in superstructures.
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hal-02025129 , version 1 (19-02-2019)

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  • HAL Id : hal-02025129 , version 1

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Jean-Charles Walter. Architecture of a bacterial DNA segregation apparatus: active caging of ParB by stochastic self-assembly nucleated from the centromere. Advanced Workshop on Interdisciplinary Views in Chromosome Structure and Function, International Centre for Theoretical Physics, Sep 2014, Trieste, Italy. ⟨hal-02025129⟩
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