Physical modeling of the ParBS complex: insight into Liquid-Liquid Phase Separation - Laboratoire Charles Coulomb (L2C) Accéder directement au contenu
Communication Dans Un Congrès Année : 2021

Physical modeling of the ParBS complex: insight into Liquid-Liquid Phase Separation

Résumé

The ParABS system is composed of three components: a centromeric sequence, parS, and two proteins, the ParA (ATPase) and the ParB DNA binding proteins. Hundreds of ParB proteins assemble dynamically to form nucleoprotein parS-anchored complexes (ParBS) that serve as substrates for ParA molecules to catalyze positioning and segregation events. We address the theoretical modeling of ParBS with a Lattice Gas models and compare it to experiments [1]. We give evidences that (i) ParBS is formed via a Liquid-Liquid Phase Separation in the metastable region of the phase diagram and (ii) this nucleation is catalyzed with the parS sequence [1]. [1] Guilhas, Walter,... & Nollmann (2020). ATP-driven separation of liquid phase condensates in bacteria. Mol. Cell, 79, 293-303.
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hal-03215528 , version 1 (03-05-2021)

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  • HAL Id : hal-03215528 , version 1

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Jean-Charles Walter. Physical modeling of the ParBS complex: insight into Liquid-Liquid Phase Separation. BioPhyChrom 2021 (The biology and physics of bacterial chromosome organization), Apr 2021, Leiden, Netherlands. ⟨hal-03215528⟩
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