Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water - Laboratoire de Biodiversité et Biotechnologies Microbiennes Accéder directement au contenu
Article Dans Une Revue Sensors Année : 2022

Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water

Selma Rabai
  • Fonction : Auteur
Ahlem Teniou
Messaoud Benounis
  • Fonction : Auteur
Jean-Louis Marty
  • Fonction : Auteur
Amina Rhouati
  • Fonction : Auteur

Résumé

Cadmium (Cd2+) is one of the most toxic heavy metals causing serious health problems; thus, designing accurate analytical methods for monitoring such pollutants is highly urgent. Herein, we report a label-free electrochemical aptasensor for cadmium detection in water. For this, a nanocomposite combining the advantages of gold nanoparticles (AuNPs), carbon nanotubes (CNTs) and chitosan (Cs) was constructed and used as immobilization support for the cadmium aptamer. First, the surface of a glassy carbon electrode (GCE) was modified with CNTs-CS. Then, AuNPs were deposited on CNTs-CS/GCE using chrono-amperometry. Finally, the immobilization of the amino-modified Cd-aptamer was achieved via glutaraldehyde cross-linking. The different synthesis steps of the AuNPs/CNTs/CS nano assembly were characterized by cyclic voltammetry (CV). Electrochemical impedance spectroscopy (EIS) was employed for cadmium determination. The proposed biosensor exhibited excellent performances for cadmium detection at a low applied potential (−0.5 V) with a high sensitivity (1.2 KΩ·M−1), a detection limit of 0.02 pM and a wide linear range (10−13–10−4 M). Moreover, the aptasensor showed a good selectivity against the interfering ions: Pb2+; Hg2+ and Zn2+. Our electrochemical biosensor provides a simple and sensitive approach for Cd2+ detection in aqueous solutions, with promising applications in the monitoring of trace amounts of heavy metals in real samples.
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Dates et versions

hal-03901782 , version 1 (04-10-2023)

Identifiants

Citer

Selma Rabai, Ahlem Teniou, Gaëlle Catanante, Messaoud Benounis, Jean-Louis Marty, et al.. Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water. Sensors, 2022, 22 (1), pp.105. ⟨10.3390/s22010105⟩. ⟨hal-03901782⟩
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