Genetic diversity and phenotypic plasticity of AHL-mediated Quorum sensing in environmental strains of Vibrio mediterranei - Laboratoire de Biodiversité et Biotechnologies Microbiennes Accéder directement au contenu
Article Dans Une Revue The International Society of Microbiologial Ecology Journal Année : 2018

Genetic diversity and phenotypic plasticity of AHL-mediated Quorum sensing in environmental strains of Vibrio mediterranei

Léa Girard
  • Fonction : Auteur
François Lantoine
  • Fonction : Auteur
Raphaël Lami
  • Fonction : Auteur
Florence Vouvé
  • Fonction : Auteur
Marcelino T Suzuki
  • Fonction : Auteur

Résumé

N-Acyl homoserine lactone (AHL)-mediated Quorum sensing (QS) is one of the most studied social behavior among Proteobacteria. However, despite the current knowledge on QS-associated phenotypes such as bioluminescence, biofilm formation, or pathogenesis, the characterization of environmental factors driving QS in realistic ecological settings remains scarce. We investigated the dynamics of AHL and AHL-producing Vibrio among 840 isolates collected fortnightly from the Salses-Leucate Mediterranean lagoon in spring and summer 2015 and 2016. Vibrio isolates were characterized by gyrB gene sequencing, Enterobacterial repetitive intergenic consensus polymerase chain reaction, and genome sequencing, and AHL production was investigated by a biosensors-based UHPLC-HRMS/MS approach. Our results revealed, for the first time, a succession of V. mediterranei isolates with different AHL production phenotypes over time and this dynamics was observed in a single genotype (average genomic nucleotide identity >99.9). A multivariate DistLM analysis revealed that 83.4% of the temporal variation of V. mediterranei QS phenotypes was explained by environmental variables. Overall, our results suggest that isolates of a single genotype are able to change their QS phenotypes in response to environmental conditions, highlighting the phenotypic plasticity of bacterial communication in the environment.
Fichier principal
Vignette du fichier
s41396-018-0260-4.pdf (901.71 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03941569 , version 1 (16-01-2023)

Identifiants

Citer

Léa Girard, François Lantoine, Raphaël Lami, Florence Vouvé, Marcelino T Suzuki, et al.. Genetic diversity and phenotypic plasticity of AHL-mediated Quorum sensing in environmental strains of Vibrio mediterranei. The International Society of Microbiologial Ecology Journal, 2018, 13 (1), pp.159 - 169. ⟨10.1038/s41396-018-0260-4⟩. ⟨hal-03941569⟩
4 Consultations
6 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More