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Communication Dans Un Congrès Année : 2022

Response of soil bacterial and hppd communities to tembotrione herbicide

Résumé

Herbicides used in agriculture aim to prevent weed growth but are known to end up in contact with soil microorganisms, thus defined as non-target organisms. Tembotrione, a recently marketed β-triketone herbicide, is known to inhibit the 4-HydroxyPhenylPyruvateDioxygenase (4-HPPD) in weeds. This enzyme is also found in numerous soil microorganisms, such as some PGPR and symbiotic bacteria, that play a key role in maintenance of ecosystem services. In this study, one of the major concerns is to assess whether tembotrione could have toxic effects on soil microorganisms and could disturb soil microbial community dynamic and structure. To investigate the possible impacts of this herbicide on these communities, a soil microcosm approach was performed using 1-fold or 10-fold the recommended tembotrione agronomical dose (RAD). Soil samples were collected at day 0, 3, 7, 14, 24, 40, and 55 to determine the following endpoints: (i) dissipation of the active ingredient in soil, (ii) bacterial diversity with high throughput sequencing of the 16S rDNA, (iii) hppd community diversity with high throughput sequencing of the hppd gene using degenerated primers designed by our own. Moreover, a culturable approach was applied to soil microcosm samples to study antibiotic resistance (ABR) of isolated soil bacteria, and to screen for HPPD+ strains. Whatever the treatment applied, tembotrione was fully dissipated from soil after 24 days at 1*RAD with a DT50 of 7 days, and after 55 days at 10*RAD with a DT50 of 15 days. Interestingly, among the culturable strains isolated from the microcosms, 29 presenting ABR, were also HPPD+. Further studies are currently performed to characterize their sensitivity to β-triketones herbicides, and their resistance to antibiotics. Finally, microbial and hppd diversity analyses with sequencing data are still ongoing at the time of writing this abstract, but results should be available by October 2022.
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Dates et versions

hal-04003276 , version 1 (24-02-2023)

Identifiants

  • HAL Id : hal-04003276 , version 1

Citer

Hugo Terol, Clémence Thiour-Mauprivez, Marion Devers-Lamrani, Fabrice Martin-Laurent, Christophe Calvayrac, et al.. Response of soil bacterial and hppd communities to tembotrione herbicide. EcotoxicoMic 2022, 3rd international conference on Microbial Ecotoxicology, Nov 2022, Montpelier, France. ⟨hal-04003276⟩
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