A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone - Laboratoire de Chimie des Processus Biologiques Accéder directement au contenu
Article Dans Une Revue Cell Chemical Biology Année : 2019

A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone

Lidia Ciccone
Yohann Couté

Résumé

Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes, remains an unresolved issue. Here, we demonstrate that seven Ubi proteins form the Ubi complex, a stable metabolon that catalyzes the last six reactions of the UQ biosynthetic pathway in Escherichia coli. The SCP2 domain of UbiJ forms an extended hydrophobic cavity that binds UQ intermediates inside the 1 MDa Ubi complex. We purify the Ubi complex from cytoplasmic extracts and demonstrate that UQ biosynthesis occurs in this fraction, challenging the current thinking of a membrane-associated biosynthetic process. Collectively, our results document a rare case of stable metabolon and highlight how the supramolecular organization of soluble enzymes allows the modification of hydrophobic substrates in a hydrophilic environment.
Fichier principal
Vignette du fichier
CELL-CHEMICAL-BIOLOGY-D-18-00418_v HAL.pdf (2.98 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02071798 , version 1 (12-11-2019)

Identifiants

Citer

Mahmoud Hajj Chehade, Ludovic Pelosi, Cameron David Fyfe, Laurent Loiseau, Bérengère Rascalou, et al.. A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone. Cell Chemical Biology, 2019, 26 (4), pp.482-492.e7. ⟨10.1016/j.chembiol.2018.12.001⟩. ⟨hal-02071798⟩
1634 Consultations
158 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More