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Article Dans Une Revue Molecular Ecology Notes Année : 2002

Isolation and characterization of microsatellites in a perennial Apiaceae, Eryngium alpinum L

Résumé

In order to investigate the genetic structure in an endangered Alpine plant (Eryngium alpinum L.), we developed microsatellites. Two different approaches were used: an enrichment protocol and the classical technique of hybridization on nylon membranes. We identified 25 loci, 13 of which revealed to be polymorphic. The polymorphism was rather low (2-6 alleles; H-E = 0.49 +/- 0.16), probably due to the short size of microsatellites (6-10 dinucleotide repeats) and to the fine spatial scale investigated. However, these markers are expected to provide a new insight about the genetic processes at work within and among E. alpinum populations.
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Dates et versions

halsde-00295041 , version 1 (11-07-2008)

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  • HAL Id : halsde-00295041 , version 1

Citer

M. Gaudeul, Y. Naciri-Graven, P. Gauthier, F. Pompanon. Isolation and characterization of microsatellites in a perennial Apiaceae, Eryngium alpinum L. Molecular Ecology Notes, 2002, 2 (2), pp.107-109. ⟨halsde-00295041⟩
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