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Article Dans Une Revue Optics Letters Année : 2004

Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos

Résumé

We demonstrate the association of third-harmonic generation (THG) microscopy and particle image velocimetry (PIV) analysis as a novel functional imaging technique for automated micrometer-scale characterization of morphogenetic movements in developing embryos. Using a combined two-photon-excited fluorescence and THG microscope, we characterize the optical properties of Drosophila embryos and show that sustained THG imaging does not perturb sensitive developmental dynamics. Velocimetric THG imaging provides a quantitative description of the dynamics of internal structures in unstained wild-type and mutant embryos.
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Dates et versions

hal-00681942 , version 1 (28-10-2013)

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Delphine Débarre, Willy Supatto, Emmanuel Farge, Bruno B. Moulia, Marie-Claire Schanne-Klein, et al.. Velocimetric third-harmonic generation microscopy: micrometer-scale quantification of morphogenetic movements in unstained embryos. Optics Letters, 2004, 29, pp.2881-2883. ⟨10.1364/OL.29.002881⟩. ⟨hal-00681942⟩
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