A role for the mevalonate pathway in early plant symbiotic signaling - Laboratoire des Interactions Plantes Microbes Environnement Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2015

A role for the mevalonate pathway in early plant symbiotic signaling

Résumé

Rhizobia and arbuscular mycorrhizal fungi produce signals that are perceived by host legume receptors at the plasma membrane and trigger sustained oscillations of the nuclear and perinuclear Ca(2+) concentration (Ca(2+) spiking), which in turn leads to gene expression and downstream symbiotic responses. The activation of Ca(2+) spiking requires the plasma membrane-localized receptor-like kinase Does not Make Infections 2 (DMI2) as well as the nuclear cation channel DMI1. A key enzyme regulating the mevalonate (MVA) pathway, 3-Hydroxy-3-Methylglutaryl CoA Reductase 1 (HMGR1), interacts with DMI2 and is required for the legume-rhizobium symbiosis. Here, we show that HMGR1 is required to initiate Ca(2+) spiking and symbiotic gene expression in Medicago truncatula roots in response to rhizobial and arbuscular mycorrhizal fungal signals. Furthermore, MVA, the direct product of HMGR1 activity, is sufficient to induce nuclear-associated Ca(2+) spiking and symbiotic gene expression in both wild-type plants and dmi2 mutants, but interestingly not in dmi1 mutants. Finally, MVA induced Ca(2+) spiking in Human Embryonic Kidney 293 cells expressing DMI1. This demonstrates that the nuclear cation channel DMI1 is sufficient to support MVA-induced Ca(2+) spiking in this heterologous system.
Fichier principal
Vignette du fichier
2015_Venkateshwaran_PNAS_1.pdf (1.34 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02631242 , version 1 (27-05-2020)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Muthusubramanian Venkateshwaran, Dhileepkumar Jayaraman, Mireille Chabaud, Andrea Genre, Allison J. Balloon, et al.. A role for the mevalonate pathway in early plant symbiotic signaling. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112 (31), pp.9781-9786. ⟨10.1073/pnas.1413762112⟩. ⟨hal-02631242⟩
14 Consultations
27 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More