Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalate (PET) Modified with Gold Nanoparticles in Flow Microchannel - Laboratoire Interfaces et Systèmes Electrochimiques Accéder directement au contenu
Article Dans Une Revue Journal of flow chemistry Année : 2014

Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalate (PET) Modified with Gold Nanoparticles in Flow Microchannel

Résumé

The real-time monitoring of the adsorption kinetic of antibody onto polyethylene terephthalate (PET) modified with gold nanoparticles (NPs) is performed into a dielectric flow microchannel. The principle is based on the microelectrode– dielectric interface excitation by a modulated voltage excitation through the dielectric layer with high frequency range. The set-up configuration using a homemade current-to-voltage converter has been developed for fast monitoring of biomolecule adsorption without direct electrical contact of microelectrodes with the microchannel flow. The change in the interfacial admittance, related to the output voltage measured through the microchip, during antibody incubation, gives information on the kinetics involved while antibodies are adsorbed at the interface. An example of adsorption on PET modified with gold NPS was taken. Experimental data were fit with the Langmuir equation and a good correlation was obtained with this latter, where the equilibrium constant, K, was found as 1.55 × 108 M−1 with a limit of detection for antibody concentration without depletion equal to 8.25 nM.

Domaines

Chimie Autre

Dates et versions

hal-01252925 , version 1 (08-01-2016)

Identifiants

Citer

Mohammed Kechadi, Mathilde Faure, Bruno Sotta, Jean Gamby. Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalate (PET) Modified with Gold Nanoparticles in Flow Microchannel. Journal of flow chemistry, 2014, 4 (2), pp.66-71. ⟨10.1556/JFC-D-13-00025⟩. ⟨hal-01252925⟩
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