Fitness advantage of Bacteroides thetaiotaomicron capsular polysaccharide is dependent on the resident microbiota - Laboratoire Jean Perrin Accéder directement au contenu
Article Dans Une Revue eLife Année : 2023

Fitness advantage of Bacteroides thetaiotaomicron capsular polysaccharide is dependent on the resident microbiota

Melanie Stäubli
  • Fonction : Auteur
Anna Sintsova
  • Fonction : Auteur
Florence Bansept
Shinichi Sunagawa
Claude Loverdo
  • Fonction : Auteur
  • PersonId : 1085703

Résumé

Many microbiota-based therapeutics rely on our ability to introduce a microbe of choice into an already-colonized intestine. However, we remain largely blind to the quantitative effects of processes determining colonization success. In this study, we used genetically-barcoded Bacteroides thetaiotaomicron ( B.theta ) strains in combination with mathematical modeling to quantify population bottlenecks experienced by B.theta during gut colonization. Integrating population bottlenecks sizes with careful quantification of net growth rates in vivo and in vitro allows us to build models describing the events during intestinal colonization in the context of gnotobiotic and complex microbiotas. Using these models, we estimated the decrease in niche size for B.theta colonization with increasing microbiota complexity. In addition, our system can be applied to mechanistically dissect colonization defects of mutant strains. As a proof of concept, we demonstrated that the competitive disadvantage of a B.theta mutant lacking capsular polysaccharide is due to a combination of an increased lag-phase before growth initiation in the gut, combined with an increased clearance rate. Crucially, the requirement for the B.theta capsule depended strongly on microbiota composition, suggesting that the dominant role may be protection from bacterial or phage aggression rather than from host-induced bactericidal mechanisms.

Dates et versions

hal-03870790 , version 1 (24-11-2022)

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Daniel Hoces, Giorgia Greter, Markus Arnoldini, Melanie Stäubli, Claudia Moresi, et al.. Fitness advantage of Bacteroides thetaiotaomicron capsular polysaccharide is dependent on the resident microbiota. eLife, 2023, 12, ⟨10.7554/eLife.81212⟩. ⟨hal-03870790⟩
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