Diatom DNA metabarcoding to assess the effect of natural radioactivity in mineral springs on ASV of benthic diatom communities - Laboratoire Microorganismes : Génome et Environnement Accéder directement au contenu
Article Dans Une Revue Science of the Total Environment Année : 2023

Diatom DNA metabarcoding to assess the effect of natural radioactivity in mineral springs on ASV of benthic diatom communities

Aude Beauger
Luc Ector

Résumé

Little is still known about the low dose effects of radiation on the microbial communities in the environment. Mineral springs are ecosystems than can be affected by natural radioactivity. These extreme environments are, therefore, observatories for studying the influence of chronic radioactivity on the natural biota. In these ecosystems we find diatoms, unicellular microalgae, playing an essential role in the food chain. The present study aimed to investigate, using DNA metabarcoding, the effect of natural radioactivity in two environmental compartments (i.e. spring sediments and water) on the genetic richness, diversity and structure of diatom communities in 16 mineral springs in the Massif Central, France. Diatom biofilms were collected during October 2019, and a 312 bp region of the chloroplast gene rbcL (coding for the Ribulose Bisphosphate Carboxylase) used as a barcode for taxonomic assignation. A total of 565 amplicon sequence variants (ASV) were found. The dominant ASV were associated with
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Dates et versions

hal-04033005 , version 1 (16-03-2023)

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Lory-Anne Baker, Aude Beauger, Sofia Kolovi, Olivier Voldoire, Elisabeth Allain, et al.. Diatom DNA metabarcoding to assess the effect of natural radioactivity in mineral springs on ASV of benthic diatom communities. Science of the Total Environment, 2023, 873, pp.162270. ⟨10.1016/j.scitotenv.2023.162270⟩. ⟨hal-04033005⟩
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