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Article Dans Une Revue Journal of Applied Microbiology Année : 2010

Rapid detection of Escherichia coli in waters using fluorescent in situ hybridization, direct viable counting and solid phase cytometry

Résumé

Aims: We developed an improved Fluorescent In Situ Hybridization FISHbased method to detect viable Escherichia coli cells by solid phase cytometry (SPC), and results were compared to those obtained by the standard culture method. Methods and Results: The method includes a direct viable count (DVC) assay, multi-probes labelled and unlabelled (helpers) to detect specifically viable E. coli cells and to enhance SPC cell counts. We demonstrate that helpers increase the fluorescence intensity of hybridized E. coli cells as detected by SPC and assess the high specificity of the DVC-FISH procedure on a large panel of cultured strains. Application to seawater, freshwater and wastewater samples showed a good correlation between SPC cells counts and standard plate counts. Conclusion: The high specificity of the procedure was demonstrated as well as its accuracy for detecting and counting viable E. coli cells in environmental samples. Significance and Impact of the Study: The developed approach may be used to monitor faecal contamination sources and to investigate the occurrence of viable E. coli in natural environments.
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Dates et versions

hal-03941897 , version 1 (16-01-2023)

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Julia Baudart, P Lebaron. Rapid detection of Escherichia coli in waters using fluorescent in situ hybridization, direct viable counting and solid phase cytometry. Journal of Applied Microbiology, 2010, 109, pp.1253 - 1264. ⟨10.1111/j.1365-2672.2010.04752.x⟩. ⟨hal-03941897⟩
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