Fluorescent Gold Nanoparticles in Suspension as an Efficient Theranostic Agent for Highly Radio-Resistant Cancer Cells - Laboratoire de Physique Corpusculaire de Clermont-Ferrand Accéder directement au contenu
Article Dans Une Revue Journal of Nanotheranostics Année : 2023

Fluorescent Gold Nanoparticles in Suspension as an Efficient Theranostic Agent for Highly Radio-Resistant Cancer Cells

Sarah Vogel
  • Fonction : Auteur
Alice O’keefe
  • Fonction : Auteur
Michael Valceski
  • Fonction : Auteur
Elette Engels
Abass Khochaiche
  • Fonction : Auteur
Carolyn Hollis
  • Fonction : Auteur
Michael Lerch
Stéphanie Corde
Christophe Massard
Komla Oscar Awitor
Moeava Tehei

Résumé

Gold nanoparticles are a promising candidate for developing new strategies of therapy against cancer. Due to their high atomic number and relative biocompatibility, they are commonly investigated as radiosensitizers to locally increase the dose of radiotherapy. In order to optimize this radiosensitizing effect, it is necessary to control the positioning of the nanoparticles in the cells. The purpose of this study is to investigate, by means of fluorescent gold nanoparticles in suspension, the dose enhancement on highly radio-resistant cancer cells. These nanoparticles were successfully produced using modern click-chemistry methods, first by attaching a chelating agent Diethylenetriamine pentaacetate benzylamine to L-cysteine, bonding the resulting ligand to a gold core, grafting propargylamine and then utilizing copper-catalyzed azide-alkyne cycloaddition (CuAAC) to fuse AlexaFluor 647 to the ligands. The results of this study prove the success of the reactions to produce a minimally cytotoxic and highly stable nanoparticle suspension that increases the radiosensitivity of gliosarcoma 9L tumor cells, with a 35% increase in cell death using 5 Gy kilovoltage radiation. Their fluorescent functionalization allowed for their simple localization within living cells and detection in vivo post-mortem.

Domaines

Chimie

Dates et versions

hal-03978120 , version 1 (08-02-2023)

Identifiants

Citer

Sarah Vogel, Alice O’keefe, Léa Seban, Michael Valceski, Elette Engels, et al.. Fluorescent Gold Nanoparticles in Suspension as an Efficient Theranostic Agent for Highly Radio-Resistant Cancer Cells. Journal of Nanotheranostics, 2023, 4 (1), pp.37-54. ⟨10.3390/jnt4010003⟩. ⟨hal-03978120⟩
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