Lignin Degradation and Its Use in Signaling Development by the Coprophilous Ascomycete Podospora anserina - Laboratoire des Sciences des Procédés et des Matériaux Accéder directement au contenu
Article Dans Une Revue Journal of Fungi Année : 2020

Lignin Degradation and Its Use in Signaling Development by the Coprophilous Ascomycete Podospora anserina

Résumé

The filamentous fungus Podospora anserina is a good model to study the breakdown of lignocellulose, owing to its ease of culture and genetical analysis. Here, we show that the fungus is able to use a wide range of lignocellulosic materials as food sources. Using color assays, spectroscopy and pyrolysis–gas chromatography mass spectrometry, we confirm that this ascomycete is able to degrade lignin, primarily by hydrolyzing β–O-4 linkages, which facilitates its nutrient uptake. We show that the limited weight loss that is promoted when attacking Miscanthus giganteus is due to a developmental blockage rather than an inefficiency of its enzymes. Finally, we show that lignin, and, more generally, phenolics, including degradation products of lignin, greatly stimulate the growth and fertility of the fungus in liquid cultures. Analyses of the CATΔΔΔΔΔ mutant lacking all its catalases, pro-oxidants and antioxidants indicate that improved growth and fertility of the fungus is likely caused by augmented reactive oxygen species levels triggered by the presence of phenolics.

Dates et versions

hal-03019337 , version 1 (23-11-2020)

Identifiants

Citer

Moussa Dicko, Roselyne Ferrari, Narumon Tangthirasunun, Valérie Gautier, Christophe Lalanne, et al.. Lignin Degradation and Its Use in Signaling Development by the Coprophilous Ascomycete Podospora anserina. Journal of Fungi, 2020, 6 (4), pp.278. ⟨10.3390/jof6040278⟩. ⟨hal-03019337⟩
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