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Poster De Conférence Année : 2015

Cellular morphometric analysis: from microscopic scale to whole mouse brains

Résumé

In neurodegenerative diseases, pathological aggregates disturb cell function and morphology. Quantifying these changes is of prime interest but raises experimental and computational challenges. In this context, whole-slide imaging (WSI) offers the unique opportunity to analyze whole mouse brain sections at the cellular level using a variety of histological markers. However, this technique generates terabytes of data which is difficult to fully analyze. We developed a novel method enabling: (1) to detect cells and pathological aggregates in WSI color images at the cellular level; (2) to quantify parameters of interest such as density, shape, location or color and (3) to integrate the information within quantitative and multi-scale heat maps. This original approach enables to extract pertinent information from high-resolution qualitative images and to dramatically reduce the amount of information to be processed. A supplementary step of this work consists in extending the analysis from brain sections to the entire brains reconstructed in 3D using our in-house software BrainVISA (http://brainvisa.info). From the generated 3D parametric maps, voxel-wise statistical studies can be performed to investigate cellular structural alterations without a priori. Furthermore, correlating 3D whole-brain parametric maps with in vivo imaging modalities (MRI, fMRI, PET, in vivo microscopy, etc.) will improve the understanding of the relationship between brain structure and function in disease.
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Dates et versions

hal-01539989 , version 1 (23-06-2017)

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  • HAL Id : hal-01539989 , version 1

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Zhenzhen You, Michel Vandenberghe, Yael Balbastre, Nicolas Souedet, Anne-Sophie Herard, et al.. Cellular morphometric analysis: from microscopic scale to whole mouse brains. 23ème Colloque Médecine et Recherche en Neurosciences de la Fondation IPSEN: "Micro-, meso- and macrodynamics", Apr 2015, Paris, France. Springer, Micro-, Meso- and Macro-Dynamics of the Brain. ⟨hal-01539989⟩
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