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Article Dans Une Revue Advanced Materials Année : 2017

Engineered Ferritin for Magnetogenetic Manipulation of Proteins and Organelles Inside Living Cells

Résumé

Magnetogenetics is emerging as a novel approach for remote-controlled manipulation of cellular functions in tissues and organisms with high spatial and temporal resolution. A critical, still challenging issue for these techniques is to conjugate target proteins with magnetic probes that can satisfy multiple colloidal and biofunctional constraints. Here, semisynthetic magnetic nanoparticles are tailored based on human ferritin coupled to monomeric enhanced green fluorescent protein (mEGFP) for magnetic manipulation of proteins inside living cells. This study demonstrates efficient delivery, intracellular stealth properties, and rapid subcellular targeting of those magnetic nanoparticles via GFP–nanobody interactions. By means of magnetic field gradients, rapid spatial reorganization in the cytosol of proteins captured to the nanoparticle surface is achieved. Moreover, exploiting efficient nanoparticle targeting to intracellular membranes, remote-controlled arrest of mitochondrial dynamics using magnetic fields is demonstrated. The studies establish subcellular control of proteins and organelles with unprecedented spatial and temporal resolution, thus opening new prospects for magnetogenetic applications in fundamental cell biology and nanomedicine.
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Dates et versions

hal-04035275 , version 1 (17-03-2023)

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Domenik Liße, Cornelia Monzel, Chiara Vicario, John Manzi, Isabelle Maurin, et al.. Engineered Ferritin for Magnetogenetic Manipulation of Proteins and Organelles Inside Living Cells. Advanced Materials, 2017, 29 (42), pp.1700189. ⟨10.1002/adma.201700189⟩. ⟨hal-04035275⟩
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