Towards concentric topology in organoids: Structuration and culture of brain derived spheroids in a Bulk Acoustic Wave resonator
Abstract
New tools and methods to control the way organoids self-organize or differentiate have recently arisen as it becomes more and more necessary to cultivate and analyze them in a reproducible manner. Here, we propose a new method using acoustic levitation to grow brain organoids from both primary neurons and patient derived glioblastomas and structure them with a concentric topology, reproducing the architecture found in vivo.
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