Demarcation of Topologically Associating Domains Is Uncoupled from Enriched CTCF Binding in Developing Zebrafish - Génétique et Biologie du Développement Accéder directement au contenu
Article Dans Une Revue iScience Année : 2020

Demarcation of Topologically Associating Domains Is Uncoupled from Enriched CTCF Binding in Developing Zebrafish

Résumé

CCCTC-binding factor (CTCF) is a conserved architectural protein that plays crucial roles in gene regulation and three-dimensional (3D) chromatin organization. To better understand mechanisms and evolution of vertebrate genome organization, we analyzed genome occupancy of CTCF in zebrafish utilizing an endogenously epitope-tagged CTCF knock-in allele. Zebrafish CTCF shares similar facets with its mammalian counterparts, including binding to enhancers, active promoters and repeat elements, and bipartite sequence motifs of its binding sites. However, we found that in vivo CTCF binding is not enriched at boundaries of topologically associating domains (TADs) in developing zebrafish, whereas TAD demarcation by chromatin marks did not differ from mammals. Our data suggest that general mechanisms underlying 3D chromatin organization, and in particular the involvement of CTCF in this process, differ between distant vertebrate species.
Fichier principal
Vignette du fichier
Perez_Rico_2020_iScience.pdf (5.76 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03983028 , version 1 (10-02-2023)

Identifiants

Citer

Yuvia Pérez-Rico, Emmanuel Barillot, Alena Shkumatava. Demarcation of Topologically Associating Domains Is Uncoupled from Enriched CTCF Binding in Developing Zebrafish. iScience, 2020, 23 (5), pp.101046. ⟨10.1016/j.isci.2020.101046⟩. ⟨hal-03983028⟩
2 Consultations
12 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More