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Pré-Publication, Document De Travail Année : 2023

A layered erythromyeloid ontogeny ensures timely supply of erythrocytes and macrophages during development

Résumé

Abstract In all organisms studied, from flies to humans, blood cells emerge in several sequential waves and from distinct hematopoietic origins. However, the relative contribution of these ontogenetically distinct hematopoietic waves to embryonic blood lineages and to tissue regeneration during development is yet elusive. Here, using a lineage-specific ‘switch and trace’ strategy in the zebrafish embryo, we report that the definitive hematopoietic progeny barely contributes to erythrocytes and macrophages during early development. Lineage tracing further show that ontogenetically distinct macrophages exhibit differential recruitment to the site of injury based on the developmental stage of the organism. We further demonstrate that primitive macrophages can solely maintain tissue regeneration during early larval developmental stages after selective ablation of definitive macrophages. Our findings highlight that the sequential emergence of hematopoietic waves in embryos ensures the abundance of blood cells required for tissue homeostasis and integrity during development Highlights Primitive hematopoietic progenitors give rise and sustain embryonic erythromyeloid lineages. Definitive hematopoietic progenitors show a late contribution to macrophages and erythrocytes during development. Embryos show a layered ontogeny of macrophages that ensures their abundance for a timely contribution to tissue homeostasis and repair. Selective ablation of definitive macrophages does not impair tail fin regeneration during early larval developmental stages. Abstract Figure
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Dates et versions

hal-04059704 , version 1 (05-04-2023)

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Ramy Elsaid, Aya Mikdache, Patricia Diabangouaya, Gwendoline Gros, Pedro P Hernández. A layered erythromyeloid ontogeny ensures timely supply of erythrocytes and macrophages during development. 2023. ⟨hal-04059704⟩
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