Lipid packing defects and membrane charge control RAB GTPase recruitment - Compartimentation et dynamique cellulaires Accéder directement au contenu
Article Dans Une Revue Traffic Année : 2018

Lipid packing defects and membrane charge control RAB GTPase recruitment

Résumé

Specific intracellular localization of RAB GTPases has been reported to be dependent on protein factors, but the contribution of the membrane physicochemical properties to this process has been poorly described. Here, we show that three RAB proteins (RAB1/RAB5/RAB6) preferentially bind in vitro to disordered and curved membranes, and that this feature is uniquely dependent on their prenyl group. Our results imply that the addition of a prenyl group confers to RAB proteins, and most probably also to other prenylated proteins, the ability to sense lipid packing defects induced by unsaturated conical-shaped lipids and curvature. Consistently, RAB recruitment increases with the amount of lipid packing defects, further indicating that these defects drive RAB membrane targeting. Membrane binding of RAB35 is also modulated by lipid packing defects but primarily dependent on negatively charged lipids. Our results suggest that a balance between hydrophobic insertion of the prenyl group into lipid packing defects and electrostatic interactions of the RAB C-terminal region with charged membranes tunes the specific intracellular localization of RAB proteins.
Fichier principal
Vignette du fichier
Kulakowski_et_al-2018-Traffic.pdf (13.07 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01829974 , version 1 (04-07-2018)

Licence

Paternité

Identifiants

Citer

Guillaume Kulakowski, Hugo Bousquet, Jean-Baptiste Manneville, Patricia Bassereau, Bruno Goud, et al.. Lipid packing defects and membrane charge control RAB GTPase recruitment. Traffic, 2018, 19 (7), pp.536-545. ⟨10.1111/tra.12568⟩. ⟨hal-01829974⟩
141 Consultations
92 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More