Evidence of phosphatidylethanolamine and phosphatidylglycerol presence at the annular region of lactose permease of Escherichia coli - Compartimentation et dynamique cellulaires Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta:Biomembranes Année : 2010

Evidence of phosphatidylethanolamine and phosphatidylglycerol presence at the annular region of lactose permease of Escherichia coli

Laura Picas
M Teresa Montero
  • Fonction : Auteur
Antoni Morros
  • Fonction : Auteur
J.L. Vázquez-Ibar
Jordi Hernández-Borrell
  • Fonction : Auteur
M. Teresa Montero
  • Fonction : Auteur

Résumé

Biochemical and structural work has revealed the importance of phospholipids in biogenesis, folding and functional modulation of membrane proteins. Therefore, the nature of protein-phospholipid interaction is critical to understand such processes. Here, we have studied the interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) mixtures with the lactose permease (LacY), the sugar/H(+) symporter from Escherichia coli and a well characterized membrane transport protein. FRET measurements between single-W151/C154G LacY reconstituted in a lipid mixture composed of POPE and POPG at different molar ratios and pyrene-labeled PE or PG revealed a different phospholipid distribution between the annular region of LacY and the bulk lipid phase. Results also showed that both PE and PG can be part of the annular region, being PE the predominant when the PE:PG molar ratio mimics the membrane of E. coli. Furthermore, changes in the thermotropic behavior of phospholipids located in this annular region confirm that the interaction between LacY and PE is stronger than that of LacY and PG. Since PE is a proton donor, the results obtained here are discussed in the context of the transport mechanism of LacY.

Domaines

Biophysique

Dates et versions

hal-02289082 , version 1 (16-09-2019)

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Citer

Laura Picas, M Teresa Montero, Antoni Morros, J.L. Vázquez-Ibar, Jordi Hernández-Borrell, et al.. Evidence of phosphatidylethanolamine and phosphatidylglycerol presence at the annular region of lactose permease of Escherichia coli. Biochimica et Biophysica Acta:Biomembranes, 2010, 1798 (2), pp.291-296. ⟨10.1016/j.bbamem.2009.06.024⟩. ⟨hal-02289082⟩
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