Role of a Kinesin Motor in Cancer Cell Mechanics - Compartimentation et dynamique cellulaires Accéder directement au contenu
Article Dans Une Revue Nano Letters Année : 2019

Role of a Kinesin Motor in Cancer Cell Mechanics

Résumé

Molecular motors play important roles in force generation, migration, and intracellular trafficking. Changes in specific motor activities are altered in numerous diseases. KIF20A, a motor protein of the kinesin-6 family, is overexpressed in bladder cancer, and KIF20A levels correlate negatively with clinical outcomes. We report here a new role for the KIF20A kinesin motor protein in intracellular mechanics. Using optical tweezers to probe intracellular mechanics and surface AFM to probe cortical mechanics, we first confirm that bladder urothelial cells soften with an increasing cancer grade. We then show that inhibiting KIF20A makes the intracellular environment softer for both high-and low-grade bladder cancer cells. Upon inhibition of KIF20A, cortical stiffness also decreases in lower grade cells, while it surprisingly increases in higher grade malignant cells. Changes in cortical stiffness correlate with the interaction of KIF20A with myosin IIA. Moreover, KIF20A inhibition negatively regulates bladder cancer cell motility irrespective of the underlying substrate stiffness. Our results reveal a central role for a microtubule motor in cell mechanics and migration in the context of bladder cancer.
Fichier principal
Vignette du fichier
nl-2019-02592e.R1_Proof_hi.pdf (4.24 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02332465 , version 1 (06-01-2021)

Identifiants

Citer

Kalpana Mandal, Katarzyna Pogoda, Satabdi Nandi, Samuel Mathieu, Amal Kasri, et al.. Role of a Kinesin Motor in Cancer Cell Mechanics. Nano Letters, 2019, 19, pp.7691-7702. ⟨10.1021/acs.nanolett.9b02592⟩. ⟨hal-02332465⟩
50 Consultations
110 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More