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Article Dans Une Revue Science Advances Année : 2021

Noninvasive quantitative assessment of collagen degradation in parchments by polarization-resolved SHG microscopy

Résumé

Nondestructive and noninvasive investigation techniques are highly sought-after to establish the degradation state of historical parchments, which is up to now assessed by thermal techniques that are invasive and destructive. We show that advanced nonlinear optical (NLO) microscopy enables quantitative in situ mapping of parchment degradation at the micrometer scale. We introduce two parameters that are sensitive to different degradation stages: the ratio of two-photon excited fluorescence to second harmonic generation (SHG) signals probes severe degradation, while the anisotropy parameter extracted from polarization-resolved SHG measurements is sensitive to early degradation. This approach is first validated by comparing NLO quantitative parameters to thermal measurements on artificially altered contemporary parchments. We then analyze invaluable parchments from the Middle Ages and show that we can map their conservation state and assess the impact of a restoration process. NLO quantitative microscopy should therefore help to identify parchments most at risk and optimize restoration methods.
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Dates et versions

hal-03289607 , version 1 (17-07-2021)

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Margaux Schmeltz, Laurianne Robinet, Sylvie Heu-Thao, Jean-Marc Sintès, Claire Teulon, et al.. Noninvasive quantitative assessment of collagen degradation in parchments by polarization-resolved SHG microscopy. Science Advances , 2021, 7 (29), pp.eabg1090. ⟨10.1126/sciadv.abg1090⟩. ⟨hal-03289607⟩
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