Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads - Laboratoire d'optique et biosciences (LOB) Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads

Résumé

To investigate the role of mechanical constraints in morphogenesis and development, we develop a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane (PDMS) mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. The elastomer rigidity after polymerization is adjusted to the tissue rigidity. Its mechanical properties are carefully calibrated in situ, for a sensor embedded in a cell aggregate submitted to uniaxial compression. The local shear stress tensor is retrieved from the sensor shape, accurately reconstructed through an active contour method. In vitro, within cell aggregates, and in vivo, in the prechordal plate of the Zebrafish embryo during gastrulation, our pipeline of techniques demonstrates its efficiency to directly measure the three dimensional shear stress repartition within a tissue, and its time evolution.
Fichier principal
Vignette du fichier
Souchaud.pdf (5.95 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03808527 , version 1 (20-10-2021)
hal-03808527 , version 2 (10-11-2022)

Identifiants

Citer

Alexandre Souchaud, Arthur Boutillon, Gaëlle Charron, Atef Asnacios, Camille Noûs, et al.. Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads. 2021. ⟨hal-03808527v1⟩
85 Consultations
82 Téléchargements

Partager

Gmail Facebook X LinkedIn More