Cdc42 and actin control polarized expression of TI-VAMP-vesicles to neuronal growth cones and their fusion with the plasma membrane. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Molecular Biology of the Cell Année : 2006

Cdc42 and actin control polarized expression of TI-VAMP-vesicles to neuronal growth cones and their fusion with the plasma membrane.

Résumé

Tetanus neurotoxin Insensitive-Vesicle Associated Membrane Protein (TI-VAMP)-mediated fusion of intracellular vesicles with the plasma membrane is crucial for neurite outgrowth, a pathway not requiring synaptobrevin-dependent exocytosis. Yet, it is not known how the TI-VAMP membrane trafficking pathway is regulated or how it is coordinated with cytoskeletal dynamics within the growth cone that guide neurite outgrowth. Here, we demonstrate that TI-VAMP, but not synaptobrevin 2, concentrates in the peripheral, F-actin-rich region of the growth cones of hippocampal neurons in primary culture. Its accumulation correlates with and depends upon the presence of F-actin. Moreover, acute stimulation of actin remodeling by homophilic activation of the adhesion molecule L1 induces a site directed, actin dependent recruitment of the TI-VAMP compartment. Expression of a dominant-positive mutant of Cdc42, a key regulator of cell polarity, stimulates formation of F-actin- and TI-VAMP-rich filopodia outside the growth cone Furthermore, we report that Cdc42 activates exocytosis of pHLuorin tagged TI-VAMP in an actin-dependent manner. Collectively, our data suggest that Cdc42 and regulated assembly of the F-actin network control the accumulation and exocytosis of TI-VAMP-containing membrane vesicles in growth cones to coordinate membrane trafficking and actin remodeling during neurite outgrowth.
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Dates et versions

hal-00016722 , version 1 (25-01-2006)

Identifiants

Citer

Philipp Alberts, Rachel Rudge, Theano Eirinopoulou, Lydia Danglot, Cécile Gauthier-Rouvière, et al.. Cdc42 and actin control polarized expression of TI-VAMP-vesicles to neuronal growth cones and their fusion with the plasma membrane.. Molecular Biology of the Cell, 2006, 17(3), pp.1194-1203. ⟨10.1091/mbc.E05-07-0643⟩. ⟨hal-00016722⟩
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