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Article Dans Une Revue Journal of the American Chemical Society Année : 2003

Electrocatalytic investigation of light-induced electron transfer between cytochrome C6 and photosystem I.

Winfried Leibl
  • Fonction : Auteur
Pierre Sétif
  • Fonction : Auteur
Hervé Bottin
  • Fonction : Auteur
Christian Bourdillon
  • Fonction : Auteur
  • PersonId : 1360650
  • IdRef : 067120393

Résumé

A light-activated electron-transfer chain was assembled using solubilized cyanobacterial photosystem I as photoactive enzyme, cytochrome c[6] (also from cyanobacteria) as electron donor, and methyl viologen as electron acceptor. The photocatalytic activity of the ensemble was measured by direct and reversible electrochemistry of cytochrome c[6] at a surface-modified gold electrode. Analysis of the electrochemical response with an appropriate model for the reaction mechanism allowed the relation of the overall catalytic reaction rate to the individual steps of the catalytic cycle. Second-order rate constants were determined for the first time under steady-state conditions. The results validate this approach as an efficient method for the study of electron transfer between photoactive enzymes and their redox partners.

Domaines

Chimie

Dates et versions

hal-00086167 , version 1 (18-07-2006)

Identifiants

Citer

Vanessa Proux-Delrouyre, Christophe Demaille, Winfried Leibl, Pierre Sétif, Hervé Bottin, et al.. Electrocatalytic investigation of light-induced electron transfer between cytochrome C6 and photosystem I.. Journal of the American Chemical Society, 2003, 125 (45), pp.13686-13692. ⟨10.1021/ja0363819⟩. ⟨hal-00086167⟩
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